Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EKLF/KLF1 Double Nickase Plasmid (h): sc-402366-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EKLF/KLF1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EKLF/KLF1 Double Nickase Plasmid (h) and EKLF/KLF1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLF1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EKLF/KLF1 Double Nickase Plasmid (h) | sc-402366-NIC | 20 µg | $410.00 | |||
EKLF/KLF1 Double Nickase Plasmid (h2) | sc-402366-NIC-2 | 20 µg | $410.00 |
Human KLF1 (EKLF) encodes an erythroid-restricted Krüppel-like transcription factor that binds CACCC elements to coordinate stage-specific gene expression during erythropoiesis. EKLF/KLF1 regulates globin locus control, erythrocyte membrane and cytoskeletal programs, heme biosynthesis, and cell-cycle exit through transcriptional networks that include BCL11A, KLF3, and erythroid maturation genes. By shaping chromatin accessibility and transcription at erythroid enhancers and promoters, KLF1 influences hemoglobin switching and red blood cell differentiation trajectories. Genetic perturbation of KLF1 is linked to altered erythroid development and hemoglobin expression patterns, supporting its relevance in modeling inherited red cell phenotypes and globin regulation mechanisms.
EKLF/KLF1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.