eIF3ζ Double Nickase Plasmid (m): sc-424946-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF3ζ Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF3ζ Double Nickase Plasmid (m) and eIF3ζ Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Eif3d. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF3ζ Antibody (H-7): sc-271515
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3ζ Double Nickase Plasmid (m) | sc-424946-NIC | 20 µg | $410.00 |
Eif3d encodes the mouse eukaryotic translation initiation factor 3 subunit zeta (eIF3ζ), a component of the eIF3 multiprotein complex that coordinates 43S preinitiation complex assembly and promotes efficient cap-dependent mRNA translation. By regulating initiation efficiency and mRNA selection, eIF3ζ influences proteome homeostasis and integrates with signaling pathways that control growth, stress adaptation, and cell-cycle progression, including mTOR-regulated translational control. Perturbation of translation initiation factors is frequently linked to altered proliferation and cellular fitness, making Eif3d a useful locus for studying dysregulated protein synthesis programs in disease-relevant contexts.
eIF3ζ Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Eif3d locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Eif3d. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Eif3d function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Eif3d-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.