Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF2Bα Double Nickase Plasmid (h): sc-404034-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF2Bα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF2Bα Double Nickase Plasmid (h) and eIF2Bα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF2B1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF2Bα Antibody (C-11): sc-376846
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bα Double Nickase Plasmid (h) | sc-404034-NIC | 20 µg | $410.00 | |||
eIF2Bα Double Nickase Plasmid (h2) | sc-404034-NIC-2 | 20 µg | $410.00 |
EIF2B1 encodes the eIF2Bα subunit of the heteropentameric eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor that regenerates eIF2-GTP to sustain cap-dependent protein synthesis. eIF2B integrates signals from the integrated stress response by modulating sensitivity to phosphorylated eIF2α, thereby linking nutrient availability, proteostasis, and cellular recovery from ER stress to translational control. Through this role, EIF2B1 influences global translation rates, stress granule dynamics, and downstream programs affecting cell growth and survival. Genetic disruption of eIF2B subunits, including EIF2B1, is associated with leukodystrophy-spectrum disorders and provides a mechanistic entry point for studying stress-adaptive translational regulation in human cells.
eIF2Bα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF2B1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF2B1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF2B1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF2B1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.