eIF2A Double Nickase Plasmid (m): sc-432849-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF2A Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF2A Double Nickase Plasmid (m) and eIF2A Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Eif2a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF2A Antibody (3A7A8): sc-517214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2A Double Nickase Plasmid (m) | sc-432849-NIC | 20 µg | $410.00 | |||
eIF2A Double Nickase Plasmid (m2) | sc-432849-NIC-2 | 20 µg | $410.00 |
Mouse Eif2a encodes eIF2A, an alternative translation initiation factor that supports initiator Met-tRNA delivery to the ribosome under conditions where canonical eIF2 activity is limited. eIF2A contributes to translational control during cellular stress, intersecting with integrated stress response signaling and selective translation programs that help remodel proteostasis. By shaping how cells initiate protein synthesis during nutrient limitation, ER stress, or other perturbations, Eif2a influences pathways governing survival, differentiation, and adaptation. Dysregulated translational initiation and stress-responsive protein synthesis are broadly relevant to neurodegeneration, metabolic dysfunction, and cancer biology, making Eif2a a useful node for mechanistic studies of stress-coupled gene expression.
eIF2A Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Eif2a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Eif2a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Eif2a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Eif2a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.