Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF1B CRISPR/Cas9 KO Plasmid (h): sc-403809
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF1B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF1B CRISPR/Cas9 KO Plasmid (h) | sc-403809 | 20 µg | $397.00 |
Overview
EIF1B encodes eukaryotic translation initiation factor 1B (eIF1B), a small initiation factor implicated in early steps of cap-dependent translation initiation and start codon selection through regulation of 40S ribosomal preinitiation complex dynamics. By influencing scanning and initiation fidelity, eIF1B contributes to proteome homeostasis and the cellular response to growth cues and stress that modulate global protein synthesis. Perturbation of translation initiation factor networks is frequently linked to altered cell proliferation programs and stress-adaptation pathways, processes commonly explored in cancer biology and neurodevelopmental research. EIF1B is therefore relevant for mechanistic studies connecting translational control to gene-expression rewiring in human cells.
eIF1B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EIF1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EIF1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EIF1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF1B protein expression.
This CRISPR knockout system enables efficient generation of EIF1B-deficient cell models for investigation of eIF1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EIF1B exon(s) critical for eIF1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EIF1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF1B CRISPR/Cas9 KO Plasmid (h) and eIF1B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EIF1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF1B HDR Plasmid (h) and eIF1B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EIF1B homology arms to support homology-directed repair at defined EIF1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.