Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EAAT1 CRISPR/Cas9 KO Plasmid (h): sc-400917
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EAAT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EAAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EAAT1 Antibody (A-3): sc-515839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EAAT1 CRISPR/Cas9 KO Plasmid (h) | sc-400917 | 20 µg | $397.00 |
Overview
SLC1A3 encodes excitatory amino acid transporter 1 (EAAT1), a high-affinity, sodium-dependent glutamate/aspartate transporter that is enriched in astrocytes and supports clearance of extracellular glutamate. By coupling glutamate uptake to ionic gradients, EAAT1 helps maintain synaptic transmission, regulates neuron–glia metabolic coupling, and limits excitotoxic signaling while contributing to redox balance through glutamate availability for glutathione synthesis. EAAT1 activity integrates with glutamatergic neurotransmission, astrocytic homeostatic programs, and pathways controlling extracellular ion buffering and neurotransmitter recycling. Altered SLC1A3 function or expression has been linked to neurodevelopmental and neurodegenerative phenotypes, including episodic ataxia and susceptibility to excitotoxic injury, making it relevant for studies of CNS circuit stability and glial biology.
EAAT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC1A3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC1A3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC1A3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EAAT1 protein expression.
This CRISPR knockout system enables efficient generation of SLC1A3-deficient cell models for investigation of EAAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC1A3 exon(s) critical for EAAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC1A3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EAAT1 CRISPR/Cas9 KO Plasmid (h) and EAAT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC1A3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EAAT1 HDR Plasmid (h) and EAAT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC1A3 homology arms to support homology-directed repair at defined SLC1A3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.