Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
E2F-2 Double Nickase Plasmid (h): sc-400880-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- E2F-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- E2F-2 Double Nickase Plasmid (h) and E2F-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting E2F2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: E2F-2 Antibody (TFE-25): sc-9967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2F-2 Double Nickase Plasmid (h) | sc-400880-NIC | 20 µg | $410.00 | |||
E2F-2 Double Nickase Plasmid (h2) | sc-400880-NIC-2 | 20 µg | $410.00 |
E2F2 encodes E2F-2, a sequence-specific transcription factor that regulates cell-cycle progression by controlling expression of genes required for G1/S transition and DNA replication. E2F-2 functions within the RB–E2F axis and integrates mitogenic and checkpoint signals to coordinate proliferation, differentiation, and apoptosis. Dysregulated E2F2 activity is implicated in oncogenic cell-cycle control, genomic instability, and altered transcriptional programs observed across multiple tumor contexts. As a node in proliferative signaling networks, E2F-2 is frequently examined in studies of replication stress, tumor suppressor pathway function, and transcriptional regulation of S-phase genes.
E2F-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the E2F2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within E2F2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt E2F2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of E2F2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.