Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
E-Selectin/CD62E/SELE CRISPR/Cas9 KO Plasmid (h): sc-400452
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- E-Selectin/CD62E/SELE CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the E-Selectin/CD62E/SELE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: E-Selectin/CD62E/SELE Antibody (D-7): sc-137054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-Selectin/CD62E/SELE CRISPR/Cas9 KO Plasmid (h) | sc-400452 | 20 µg | $397.00 |
Overview
SELE encodes E-selectin (CD62E), an inducible endothelial cell adhesion molecule rapidly upregulated by inflammatory cytokines such as TNF-α and IL-1β. By binding sialylated, fucosylated ligands (including sialyl-Lewis^x) on leukocytes, E-selectin mediates tethering and rolling on activated endothelium, initiating the leukocyte adhesion cascade and transendothelial migration. SELE function intersects with NF-κB–driven inflammatory signaling and regulates vascular leukocyte recruitment that shapes tissue inflammation and immune surveillance. Dysregulated E-selectin expression is associated with chronic inflammatory states and vascular pathology, and is frequently studied in tumor–endothelial interactions and metastasis-relevant extravasation models.
E-Selectin/CD62E/SELE CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SELE gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SELE together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SELE open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish E-Selectin/CD62E/SELE protein expression.
This CRISPR knockout system enables efficient generation of SELE-deficient cell models for investigation of E-Selectin/CD62E/SELE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SELE exon(s) critical for E-Selectin/CD62E/SELE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SELE genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by E-Selectin/CD62E/SELE CRISPR/Cas9 KO Plasmid (h) and E-Selectin/CD62E/SELE CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SELE locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by E-Selectin/CD62E/SELE HDR Plasmid (h) and E-Selectin/CD62E/SELE HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SELE homology arms to support homology-directed repair at defined SELE target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.