Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynamin I Double Nickase Plasmid (h): sc-401183-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynamin I Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynamin I Double Nickase Plasmid (h) and Dynamin I Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynamin I Antibody (D5): sc-12724
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynamin I Double Nickase Plasmid (h) | sc-401183-NIC | 20 µg | $410.00 | |||
Dynamin I Double Nickase Plasmid (h2) | sc-401183-NIC-2 | 20 µg | $410.00 |
DNM1 encodes Dynamin I, a neuron-enriched large GTPase that catalyzes membrane fission during clathrin-mediated endocytosis and synaptic vesicle recycling. Through GTP-dependent oligomerization at the neck of budding vesicles, Dynamin I coordinates with endocytic adaptors and actin-regulatory factors to maintain vesicle trafficking, neurotransmitter release, and membrane homeostasis. DNM1 activity integrates into pathways governing synaptic transmission, vesicular transport, and membrane remodeling, with perturbations linked to neurodevelopmental and epileptic phenotypes. Dysregulated endocytosis and synaptic vesicle dynamics associated with DNM1 provide a mechanistic entry point for studying neuronal excitability, network function, and trafficking-dependent proteostasis.
Dynamin I Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.