Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynactin 6 CRISPR/Cas9 KO Plasmid (h): sc-406516
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynactin 6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dynactin 6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynactin 6 Antibody (B-6): sc-398694
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynactin 6 CRISPR/Cas9 KO Plasmid (h) | sc-406516 | 20 µg | $397.00 |
Overview
DCTN6 encodes dynactin subunit 6, a component of the dynactin complex that cooperates with cytoplasmic dynein to support microtubule-based transport. Dynactin helps link dynein to diverse cargos and contributes to intracellular trafficking, vesicle motility, and cytoskeletal organization that influence organelle positioning and mitotic processes. Through these roles, Dynactin 6 can affect pathways governing endosome–lysosome dynamics, centrosome function, and cell division fidelity. Perturbation of dynein–dynactin–dependent transport has been associated with neurological and proliferative phenotypes, making DCTN6 relevant to studies of cellular homeostasis and disease mechanisms involving trafficking defects.
Dynactin 6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCTN6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCTN6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCTN6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dynactin 6 protein expression.
This CRISPR knockout system enables efficient generation of DCTN6-deficient cell models for investigation of Dynactin 6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCTN6 exon(s) critical for Dynactin 6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCTN6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dynactin 6 CRISPR/Cas9 KO Plasmid (h) and Dynactin 6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCTN6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dynactin 6 HDR Plasmid (h) and Dynactin 6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCTN6 homology arms to support homology-directed repair at defined DCTN6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.