Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DUSP18 CRISPR/Cas9 KO Plasmid (h): sc-405897
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUSP18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DUSP18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DUSP18 Antibody (E-2): sc-376923
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUSP18 CRISPR/Cas9 KO Plasmid (h) | sc-405897 | 20 µg | $397.00 |
Overview
Dual specificity phosphatase 18 (DUSP18) is a member of the atypical DUSP family that dephosphorylates phosphoserine/threonine and phosphotyrosine residues to modulate kinase-driven signaling. It has been linked to regulation of MAPK pathway tone and broader cellular stress-response processes that influence proliferation, survival, and mitochondrial function. By shaping phosphorylation-dependent signaling networks, DUSP18 can affect transcriptional programs and metabolic homeostasis in a context-dependent manner. Altered phosphatase activity in this class of regulators is relevant to diseases characterized by dysregulated signaling, including cancer and inflammatory or metabolic phenotypes, making DUSP18 a useful node for pathway interrogation.
DUSP18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUSP18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUSP18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUSP18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DUSP18 protein expression.
This CRISPR knockout system enables efficient generation of DUSP18-deficient cell models for investigation of DUSP18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUSP18 exon(s) critical for DUSP18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUSP18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DUSP18 CRISPR/Cas9 KO Plasmid (h) and DUSP18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUSP18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DUSP18 HDR Plasmid (h) and DUSP18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUSP18 homology arms to support homology-directed repair at defined DUSP18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.