Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Doublecortin/DCX CRISPR/Cas9 KO Plasmid (h): sc-400033
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Doublecortin/DCX CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Doublecortin/DCX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Doublecortin/DCX Antibody (E-6): sc-271390
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Doublecortin/DCX CRISPR/Cas9 KO Plasmid (h) | sc-400033 | 20 µg | $397.00 |
Overview
DCX encodes doublecortin, a microtubule-associated protein that binds and stabilizes polymerized microtubules to coordinate neuronal migration, neurite outgrowth, and cortical layer formation during neurodevelopment. Doublecortin functions within cytoskeletal remodeling programs that integrate microtubule dynamics with centrosome positioning and growth cone guidance, influencing polarization and axon extension in developing neurons. Pathogenic variation or dysregulation of DCX is linked to neurodevelopmental disorders characterized by disrupted cortical organization, including X-linked lissencephaly and subcortical band heterotopia. As a cell biology marker of migrating neuroblasts and immature neurons, DCX is widely used to study developmental trajectories and mechanisms governing neuronal positioning.
Doublecortin/DCX CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Doublecortin/DCX protein expression.
This CRISPR knockout system enables efficient generation of DCX-deficient cell models for investigation of Doublecortin/DCX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCX exon(s) critical for Doublecortin/DCX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Doublecortin/DCX CRISPR/Cas9 KO Plasmid (h) and Doublecortin/DCX CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Doublecortin/DCX HDR Plasmid (h) and Doublecortin/DCX HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCX homology arms to support homology-directed repair at defined DCX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.