Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DNA Ligase III Double Nickase Plasmid (h): sc-402841-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNA Ligase III Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DNA Ligase III Double Nickase Plasmid (h) and DNA Ligase III Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LIG3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DNA Ligase III Antibody (E-7): sc-390922
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA Ligase III Double Nickase Plasmid (h) | sc-402841-NIC | 20 µg | $410.00 | |||
DNA Ligase III Double Nickase Plasmid (h2) | sc-402841-NIC-2 | 20 µg | $410.00 |
LIG3 encodes human DNA ligase III, an ATP-dependent ligase that seals single-strand breaks during base excision repair and coordinates repair of oxidative DNA damage through interactions with XRCC1. DNA ligase III also contributes to alternative end joining and participates in genome maintenance processes that protect replication forks and preserve chromosomal stability. Its mitochondrial isoform supports mitochondrial DNA repair and integrity, linking LIG3 function to cellular bioenergetics and stress responses. Dysregulation or loss of LIG3 activity is associated with elevated DNA damage signaling, increased mutation burden, and genomic instability phenotypes relevant to cancer biology and neurodegeneration research.
DNA Ligase III Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LIG3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LIG3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LIG3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LIG3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.