demethylated-PP2A-C Antibody (4i57): sc-80990

Demethylated-PP2A-C Antibody (4i57) is a mouse monoclonal IgG1 antibody that recognizes demethylated-PP2A-C in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Demethylated-PP2A-C plays a crucial role in cellular signaling pathways by regulating protein activity through dephosphorylation, which maintains cellular homeostasis and controls processes like cell division and apoptosis. Demethylated-PP2A-C is primarily located in the cytoplasm and nucleus, where anti-demethylated-PP2A-C antibody (4i57) can detect interactions with regulatory subunits, including PP2A-Aα, PP2A-Bα, and PP2A-B56α, forming a functional holoenzyme complex. This complex modulates target protein phosphorylation states, influencing numerous cellular functions and responses to external stimuli. Demethylated-PP2A-C′s regulation of these processes highlights its importance in both normal physiology and disease states, including cancer, where dysregulation of phosphatase activity can lead to aberrant signaling pathways. Demethylated-PP2A-C (4i57) antibody serves as an invaluable tool for researchers investigating protein phosphatases in cellular regulation and disease mechanisms.

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demethylated-PP2A-C Antibody (4i57) References:

1. Parallel purification of three catalytic subunits of the protein serine/threonine phosphatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with alpha4 protein.  |  Kloeker, S., et al. 2003. Protein Expr Purif. 31: 19-33. PMID: 12963337
2. PPP2R1B gene alterations inhibit interaction of PP2A-Abeta and PP2A-C proteins in colorectal cancers.  |  Tamaki, M., et al. 2004. Oncol Rep. 11: 655-9. PMID: 14767517
3. Silencing of the PP2A catalytic subunit causes HER-2/neu positive breast cancer cells to undergo apoptosis.  |  Wong, LL., et al. 2010. Exp Cell Res. 316: 3387-96. PMID: 20558158
4. The effect of deleting residue C269 in the β12-β13 loop of protein phosphatase 2A (PP2A)catalytic subunit on the interaction between PP2A and metal ions, especially Mn(2+).  |  Li, H., et al. 2011. Biochim Biophys Acta. 1814: 1769-74. PMID: 21996587
5. Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit in vitro.  |  Li, JY., et al. 2012. Acta Pharmacol Sin. 33: 935-40. PMID: 22684030
6. Hepatocyte-specific ablation of PP2A catalytic subunit α attenuates liver fibrosis progression via TGF-β1/Smad signaling.  |  Lu, N., et al. 2015. Biomed Res Int. 2015: 794862. PMID: 25710025
7. Quantitative proteomics reveals novel protein interaction partners of PP2A catalytic subunit in pancreatic β-cells.  |  Zhang, X., et al. 2016. Mol Cell Endocrinol. 424: 1-11. PMID: 26780722
8. MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.  |  Shi, Q., et al. 2017. Mol Immunol. 88: 79-88. PMID: 28609714
9. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation.  |  Frohner, IE., et al. 2020. Cell Rep. 30: 3171-3182.e6. PMID: 32130916
10. lncRNA SNHG1 Promotes Basal Bladder Cancer Invasion via Interaction with PP2A Catalytic Subunit and Induction of Autophagy.  |  Xu, J., et al. 2020. Mol Ther Nucleic Acids. 21: 354-366. PMID: 32650234

The preferred demethylated-PP2A-C antibody is demethylated-PP2A-C Antibody (4B7): sc-13601.