DDX34 Double Nickase Plasmid (h): sc-411318-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX34 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX34 Double Nickase Plasmid (h) and DDX34 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHX34. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX34 Antibody (E-3): sc-514665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX34 Double Nickase Plasmid (h) | sc-411318-NIC | 20 µg | $410.00 | |||
DDX34 Double Nickase Plasmid (h2) | sc-411318-NIC-2 | 20 µg | $410.00 |
DHX34 (DDX34) encodes a conserved DEAH-box RNA helicase that participates in post-transcriptional RNA metabolism and regulation of mRNA surveillance. It is linked to nonsense-mediated mRNA decay (NMD) and RNA processing events that influence transcript stability and quality control, thereby shaping gene expression programs during cell growth and differentiation. Perturbation of DHX34 activity can shift RNA homeostasis and stress-response signaling, making it relevant for studying pathways that connect RNA surveillance to genome integrity and cellular fitness. Altered DHX34 expression or function has been reported in molecular studies of malignancy-associated transcriptional states, supporting its use as a mechanistic node in disease-relevant RNA regulatory networks.
DDX34 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHX34 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHX34. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHX34 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHX34-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.