DDX23 CRISPR/Cas9 KO Plasmid (h): sc-407132
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX23 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX23 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX23 CRISPR/Cas9 KO Plasmid (h) | sc-407132 | 20 µg | $397.00 |
Overview
DDX23 encodes a DEAD-box RNA helicase that functions as a core component of the U5 snRNP within the spliceosome, where it helps remodel RNA–protein complexes to support pre-mRNA splicing. By regulating splice site recognition and spliceosomal dynamics, DDX23 influences transcript maturation, isoform balance, and downstream gene expression programs linked to cell-cycle progression and stress responses. Perturbation of spliceosome-associated helicases can shift alternative splicing patterns and RNA processing fidelity, processes frequently implicated in oncogenic transcriptional rewiring and genome instability. As a result, DDX23 is commonly studied in the context of RNA metabolism, proteostasis, and pathways coupling splicing to DNA damage signaling.
DDX23 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDX23 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX23 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX23 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX23 protein expression.
This CRISPR knockout system enables efficient generation of DDX23-deficient cell models for investigation of DDX23 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX23 exon(s) critical for DDX23 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX23 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX23 CRISPR/Cas9 KO Plasmid (h) and DDX23 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX23 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX23 HDR Plasmid (h) and DDX23 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX23 homology arms to support homology-directed repair at defined DDX23 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.