DcR2 Double Nickase Plasmid (h): sc-403389-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DcR2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DcR2 Double Nickase Plasmid (h) and DcR2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFRSF10D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DcR2 Antibody (B-P30): sc-65310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DcR2 Double Nickase Plasmid (h) | sc-403389-NIC | 20 µg | $410.00 | |||
DcR2 Double Nickase Plasmid (h2) | sc-403389-NIC-2 | 20 µg | $410.00 |
TNFRSF10D encodes decoy receptor 2 (DcR2), a TNF receptor superfamily member that binds TRAIL (TNFSF10) but lacks a functional cytoplasmic death domain, thereby modulating extrinsic apoptosis signaling at the cell surface. By competing with death receptors such as DR4 and DR5 for ligand engagement, DcR2 can reshape downstream caspase activation, NF-κB signaling outputs, and stress-response programs that influence cell fate decisions. DcR2 is also linked to senescence-associated phenotypes and inflammatory signaling contexts where TRAIL pathway balance affects tissue homeostasis. Altered TNFRSF10D expression has been reported in multiple disease-relevant settings, supporting its use as a mechanistic node for studying apoptosis resistance and immune-related signaling networks.
DcR2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFRSF10D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFRSF10D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFRSF10D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFRSF10D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.