Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DCAMKL1 Double Nickase Plasmid (h): sc-402160-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DCAMKL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DCAMKL1 Double Nickase Plasmid (h) and DCAMKL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCLK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DCAMKL1 Antibody (D-3): sc-514684
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DCAMKL1 Double Nickase Plasmid (h) | sc-402160-NIC | 20 µg | $410.00 | |||
DCAMKL1 Double Nickase Plasmid (h2) | sc-402160-NIC-2 | 20 µg | $410.00 |
DCLK1 (DCAMKL1) encodes a microtubule-associated serine/threonine kinase that integrates doublecortin-like microtubule binding with kinase signaling to regulate cytoskeletal dynamics, neuronal migration, and neurite outgrowth. In epithelial contexts, DCLK1 has been used as a marker of tuft cells and is linked to programs governing cell fate, motility, and stress-adaptive signaling. Reported pathway associations include microtubule remodeling, MAPK-related signaling, and regulation of transcriptional states coupled to differentiation. Dysregulated DCLK1 expression has been described in multiple tumor types and inflammatory settings, supporting research into mechanisms of stem-like phenotypes, invasion, and microenvironmental interactions without implying clinical outcomes.
DCAMKL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCLK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCLK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCLK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCLK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.