Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DAZAP2 CRISPR/Cas9 KO Plasmid (h): sc-407056
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAZAP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DAZAP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAZAP2 Antibody (G-4): sc-515182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAZAP2 CRISPR/Cas9 KO Plasmid (h) | sc-407056 | 20 µg | $397.00 |
Overview
DAZAP2 (DAZ-associated protein 2) is an adaptor-like cytoplasmic protein implicated in integrating receptor-proximal signals with downstream transcriptional and cytoskeletal responses. It has been linked to modulation of major signaling cascades including Wnt/β-catenin and MAPK/ERK, influencing cellular proliferation, differentiation, and context-dependent transcriptional programs. DAZAP2 also associates with components involved in RNA and protein regulatory networks, supporting roles in post-transcriptional control and signal-dependent protein interactions. Altered DAZAP2 expression or pathway engagement has been reported across multiple cancer-related datasets, making it relevant for studying oncogenic signaling rewiring and cell-state transitions in human model systems.
DAZAP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DAZAP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DAZAP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DAZAP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DAZAP2 protein expression.
This CRISPR knockout system enables efficient generation of DAZAP2-deficient cell models for investigation of DAZAP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DAZAP2 exon(s) critical for DAZAP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DAZAP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DAZAP2 CRISPR/Cas9 KO Plasmid (h) and DAZAP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DAZAP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DAZAP2 HDR Plasmid (h) and DAZAP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DAZAP2 homology arms to support homology-directed repair at defined DAZAP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.