DAP12 Double Nickase Plasmid (m): sc-423568-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAP12 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DAP12 Double Nickase Plasmid (m) and DAP12 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tyrobp. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAP12 Antibody (A-4): sc-166084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAP12 Double Nickase Plasmid (m) | sc-423568-NIC | 20 µg | $410.00 | |||
DAP12 Double Nickase Plasmid (m2) | sc-423568-NIC-2 | 20 µg | $410.00 |
Tyrobp encodes DAP12 (TYROBP), a transmembrane adaptor bearing an immunoreceptor tyrosine-based activation motif (ITAM) that couples multiple activating receptors in myeloid cells to intracellular signaling. In mouse microglia, macrophages, dendritic cells, and osteoclast lineage cells, DAP12 associates with receptors such as TREM2, SIRPβ1, and other Ig-like receptors to promote SYK-dependent cascades that regulate phagocytosis, cytokine production, chemotaxis, survival, and cytoskeletal remodeling. This signaling axis intersects with innate immune pathways including FcR-mediated responses and PI3K–AKT and MAPK signaling, shaping inflammatory tone and tissue surveillance. Altered TYROBP/DAP12 network activity is widely studied in contexts such as neuroinflammation and microglial state transitions, host defense, and bone remodeling disorders.
DAP12 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tyrobp locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tyrobp. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tyrobp function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tyrobp-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.