Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 14 Double Nickase Plasmid (h): sc-400362-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cytokeratin 14 Double Nickase Plasmid (h) and Cytokeratin 14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KRT14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 14 Antibody (LL001): sc-53253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 14 Double Nickase Plasmid (h) | sc-400362-NIC | 20 µg | $410.00 | |||
Cytokeratin 14 Double Nickase Plasmid (h2) | sc-400362-NIC-2 | 20 µg | $410.00 |
KRT14 encodes cytokeratin 14, a type I intermediate filament protein that heterodimerizes with keratin 5 to form the basal keratin network in stratified epithelia. This filament system supports mechanical resilience, organizes desmosome and hemidesmosome adhesion complexes, and influences keratinocyte polarity, migration, and stress responses during epidermal homeostasis and wound repair. KRT14 is a canonical marker of basal keratinocytes and is frequently used to study epithelial differentiation programs and cytoskeletal remodeling. Altered KRT14 expression or pathogenic variants are linked to epidermal fragility disorders and are leveraged as molecular readouts in models of skin barrier dysfunction and squamous epithelial pathobiology.
Cytokeratin 14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KRT14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KRT14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KRT14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KRT14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.