COL9A1 Double Nickase Plasmid (h): sc-405807-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL9A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL9A1 Double Nickase Plasmid (h) and COL9A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL9A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL9A1 Antibody (H-7): sc-376969
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL9A1 Double Nickase Plasmid (h) | sc-405807-NIC | 20 µg | $410.00 | |||
COL9A1 Double Nickase Plasmid (h2) | sc-405807-NIC-2 | 20 µg | $410.00 |
COL9A1 encodes the alpha-1 chain of type IX collagen, a FACIT collagen that decorates type II collagen fibrils and contributes to the structural organization of hyaline cartilage and other extracellular matrix-rich tissues. Through its interactions with fibrillar collagens and proteoglycans, COL9A1 supports matrix assembly, fibril stability, and biomechanical properties important for chondrocyte homeostasis. COL9A1 activity is linked to extracellular matrix organization and cartilage development processes that intersect with connective tissue remodeling pathways. Genetic disruption or altered expression of COL9A1 has been associated with cartilage degeneration phenotypes and inherited chondrodysplasias, supporting its relevance for studies of skeletal biology and joint disease mechanisms.
COL9A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL9A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL9A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL9A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL9A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.