Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COL23A1 CRISPR/Cas9 KO Plasmid (h): sc-413896
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL23A1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL23A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL23A1 Antibody (C-10): sc-514835
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL23A1 CRISPR/Cas9 KO Plasmid (h) | sc-413896 | 20 µg | $397.00 |
Overview
COL23A1 encodes collagen type XXIII alpha 1, a transmembrane collagen enriched at the cell surface where it contributes to extracellular matrix organization and cell–matrix adhesion. By influencing interactions with basement membrane components and integrin-associated complexes, COL23A1 can modulate processes linked to epithelial architecture, migration, and tissue remodeling. Altered COL23A1 expression has been reported in multiple cancer- and fibrosis-related contexts, supporting its utility as a marker and mechanistic node in studies of invasion, metastatic potential, and stromal remodeling. As a membrane-associated collagen, it provides a tractable entry point for investigating how pericellular ECM cues shape signaling and cellular behavior.
COL23A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL23A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL23A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL23A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL23A1 protein expression.
This CRISPR knockout system enables efficient generation of COL23A1-deficient cell models for investigation of COL23A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL23A1 exon(s) critical for COL23A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL23A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL23A1 CRISPR/Cas9 KO Plasmid (h) and COL23A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL23A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL23A1 HDR Plasmid (h) and COL23A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL23A1 homology arms to support homology-directed repair at defined COL23A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.