Date published: 2025-9-7

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COL1A1 Anticorpo (3G3): sc-293182

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Fichas de dados
  • COL1A1 Anticorpo 3G3é um anticorpo monoclonal produzido em camundongo IgG3 κ COL1A1 anticorpo, citado em 179 publicações, fornecido em 100 µg/ml
  • elevada contra uma proteína recombinante correspondente aos aminoácidos 1021-1108 de COL1A1 de origem human
  • recomendado para a detecção de Collagen α1 Type I de mouse, rat e human origem em
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de COL1A1 (3G3): sc-293182.
  • m-IgG3 BP-HRP é o reagente de deteção secundária preferido para COL1A1 Anticorpo (3G3) para aplicações WB and IHC(P). Este reagente é agora oferecido num pacote com COL1A1 Anticorpo (3G3) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O anticorpo COL1A1 (3G3) é um anticorpo monoclonal de cadeia leve IgG3 kappa de camundongo que tem como alvo o colagénio α1 tipo I, também conhecido como COL1A1 ou cadeia alfa-1 do colagénio tipo I, um componente vital da matriz extracelular (ECM) em vários tecidos conjuntivos. O anticorpo monoclonal COL1A1 (3G3) foi criado contra uma proteína recombinante correspondente aos aminoácidos 1021-1108 do COL1A1 humano, detectando o COL1A1 em amostras de camundongo, rato e humano através de aplicações como western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF), imunohistoquímica com secções incluídas em parafina (IHCP) e ensaio de imunoabsorção enzimática (ELISA). A COL1A1 está predominantemente localizada na MEC, onde forma estruturas fibrilares que proporcionam resistência à tração e integridade estrutural a tecidos como tendões, pele e osso. A presença de COL1A1 na MEC é essencial para manter a arquitetura dos tecidos, facilitar a adesão celular e apoiar funções celulares como a migração e a diferenciação. Além disso, a COL1A1 desempenha um papel importante na cicatrização de feridas e na reparação de tecidos, contribuindo para a formação de tecido cicatricial. O anticorpo anti-COL1A1 (3G3) está disponível numa forma não conjugada, permitindo aos investigadores incorporá-lo em vários protocolos experimentais destinados a estudar processos relacionados com o colagénio. O anticorpo monoclonal COL1A1 (3G3) é recomendado para investigadores que estudam a dinâmica da matriz extracelular e as suas implicações na saúde e na doença.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do COL1A1 Anticorpo (3G3):

  1. Colagénio e resistência óssea.  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. Artigo de revisão: interações plaquetas-colagénio: receptores de membrana e vias de sinalização intracelular.  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. Adesão celular a matrizes colagénicas.  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  4. Tolerância nasal aos epítopos dominantes e subdominantes do colagénio tipo II e proteção contra a artrite induzida pelo colagénio.  |  Staines, NA., et al. 1997. Biochem Soc Trans. 25: 661-4. PMID: 9191176
  5. Colagénios versáteis em invertebrados.  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  6. Artrite induzida por colagénio, um modelo animal de autoimunidade.  |  Myers, LK., et al. 1997. Life Sci. 61: 1861-78. PMID: 9364191
  7. Os colagénios da cartilagem: uma revisão da sua estrutura, organização e papel na patogénese da artrite experimental em animais e na doença reumática humana.  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

COL1A1 Anticorpo (3G3)

sc-293182
100 µg/ml
$316.00

Pacote do COL1A1 (3G3): m-IgG3 BP-HRP

sc-550413
100 µg Ab; 40 µg BP
$354.00

I understand that this antibody is raised against human col1a1 amino acid #1021-1108. What about the amino acid# of mouse col1a1?

Asked by: Sr99
Thank you for your question. Please see link for more information on this antibody : https://www.uniprot.org/uniprotkb/P11087/entry
Answered by: BlakeJ
Date published: 2024-04-06

Do you recommend use milk or BSA with this antibody?

Asked by: MDias
Thank you for your question. This antibody has been established with UltraCruz® Blocking Reagent: sc-516214. BSA-TBST or milk-TBST are also suitable for blocking. Depending on your particular experimental setup you may want to opt for a BSA-based blocking buffer to increase overall signal strength or a milk-based buffer for improved signal to noise ratio and lower background.
Answered by: Tech Support Europe
Date published: 2023-10-24

Is it possible to use this antibody for IHC(P) on rabbit tissue?

Asked by: XuMY
Thank you for your question. It doesn't seem COL1A1 is fully characterized in Oryctolagus cuniculus. However, there seems to be a high homology between the epitope sequence of the antibody and the predicted sequence of collagen alpha-1(I) chain of rabbit origin, accession# XP_017204320. It may be possible that this antibody may detect your target but we cannot guarantee it since the sequence has not been fully characterized.
Answered by: Santa Cruz TS
Date published: 2021-07-10

Would this antibody work on COL1 of porcine origin?

Asked by: KaraThrace
Thank you for your question. This antibody has not yet been tested in porcine and so that is not a recommended species at this time. We are continuously gathering data to show on our website. Check back for potential updates! If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-12

Would this ABY work for western analysis of the purified rat-tail collagen used for cell culture?

Asked by: Monger
Thank you for your question. We haven't tested this antibody using purified rat-tail collagen used for cell culture, so we do not know if it would work on that protein.
Answered by: Technical Support
Date published: 2022-06-01

I checked col1a1 in mice kidney extract through WB.While Band positions were detected from 52 kDa to 76 kDa, not in the same location as the protocol. Even if it is a decomposed product, the molecular weight is small. Please tell me What is it.

Asked by: MHTK
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-23

hello. I have an one question about this antibody. Can this antibody even recognize pro collagen type 1? I'm wondering if this ab can distinguish collagen fiber and pro collagen.

Asked by: finalb2
Thank you for your question. Based on the epitope mapping, this antibody should be able to recognize both pro collagen alpha1 type I, and the mature protein. We have not tested the antibody to determine if it can distinguish between collagen fiber and pro collagen, but we would recommend you review some of the product citations using this antibody for more information about how the antibody is being used.
Answered by: Technical Support
Date published: 2020-03-31

I used this antibody to check col1a1 in mice kidney extract through WB.While the reasult showed a lot of non-specific bands and i couldn't comfirm which is the col1a1. 1st dil. 1:1000. 2nd anti-mouse lgG dil.1:5000. Could u provide a positive con?

Asked by: hhx112
Thank you for your question. Please contact your local distributor for assistance in troubleshooting this antibody. They will be happy to help you!
Answered by: Tech Service
Date published: 2021-03-19
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Rated 4 out of 5 by from Buon funzionamento su FFPETestato su cute canina 1:150, buon funzionamento, un po' di fondo.
Date published: 2025-05-12
Rated 5 out of 5 by from Very good bands in Western blottingBoth mature collagen and procollagen can be detected, although it takes some trickery to solubilize samples for collagen-specific protocol.
Date published: 2024-08-18
Rated 2 out of 5 by from Reduced visibility and aspecificity in WBI tested this antibody on NHDF human dermal fibroblasts, to evaluate the modulation of collagen under the modulators of the Wound healing process. However, in its application for western blot, using the 1:1000 dilution, the antibody highlights a non-specific around 50 kDa, the intensity of which reduces the visibility of the isoform for which this antibody should be specific. I recommend cutting the membrane properly.
Date published: 2023-03-27
Rated 4 out of 5 by from Col1A1 in conditioned mediaWe used the Col1A1 antibody in human lung fibroblast culture treated with TGF-b1. Supernatant were collected 24, hours. Slot blot analysis revealed increase in Col1A1 expression.
Date published: 2022-06-06
Rated 4 out of 5 by from Bene in wbHo provato questo anticorpo in wb (1:1000) su spinal cord di topo ed ha funzionato molto bene! Ottimo anticorpo
Date published: 2020-10-05
Rated 5 out of 5 by from Good for WBIt's useful for detecting both Pro-collagen I and matured collagen I.
Date published: 2020-09-04
Rated 5 out of 5 by from good for immunohistochemistryImmunohistochemistry: Paraffin kidney section, immune infiltrate Antibody dilution: 1:50 Antigen retrieval in citrate buffer pH6 Comment: Good signal
Date published: 2020-08-10
Rated 1 out of 5 by from No encontramos señal.WB muy sucio; imposible detectar señal de ñaproteina.
Date published: 2020-05-09
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COL1A1 Anticorpo (3G3) is rated 4.1 out of 5 by 29.
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