Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CNPase CRISPR/Cas9 KO Plasmid (h): sc-402463
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNPase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CNPase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNPase Antibody (H-2): sc-166558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNPase CRISPR/Cas9 KO Plasmid (h) | sc-402463 | 20 µg | $397.00 |
Overview
CNP encodes 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), a myelin-associated enzyme enriched in oligodendrocytes that hydrolyzes 2',3'-cyclic nucleotides and contributes to myelin sheath formation and maintenance. CNPase localizes to non-compact myelin and cytoskeletal/membrane microdomains, where it is linked to RNA metabolism, process outgrowth, and regulation of oligodendroglial morphology. Through these functions, CNPase supports axon–glia interactions and myelin stability, intersecting with pathways governing glial differentiation and neuroinflammatory responses. Altered CNP expression or CNPase activity has been associated with demyelinating and neurodegenerative contexts, making it a useful target for mechanistic studies of white matter biology.
CNPase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CNP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CNPase protein expression.
This CRISPR knockout system enables efficient generation of CNP-deficient cell models for investigation of CNPase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CNP exon(s) critical for CNPase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CNP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CNPase CRISPR/Cas9 KO Plasmid (h) and CNPase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CNPase HDR Plasmid (h) and CNPase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNP homology arms to support homology-directed repair at defined CNP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.