Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h): sc-400863-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h) and Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLTC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clathrin Heavy Chain/CLTC Antibody (TD.1): sc-12734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h) | sc-400863-NIC | 20 µg | $410.00 | |||
Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h2) | sc-400863-NIC-2 | 20 µg | $410.00 |
CLTC encodes the clathrin heavy chain, a core structural component of clathrin-coated pits and vesicles that mediates receptor internalization and membrane trafficking between the plasma membrane, endosomes, and the trans-Golgi network. By assembling into polyhedral lattices with adaptor proteins, CLTC coordinates cargo selection and vesicle budding, supporting endocytosis, synaptic vesicle recycling, and regulated turnover of signaling receptors. These processes intersect with pathways controlling growth factor signaling, nutrient uptake, and organelle homeostasis. Dysregulated clathrin-mediated transport has been linked to altered receptor signaling and genomic rearrangements involving CLTC in certain malignancies, making CLTC a useful node for studying trafficking-dependent phenotypes in human cells.
Clathrin Heavy Chain/CLTC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLTC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLTC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLTC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLTC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.