Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CHDH CRISPR/Cas9 KO Plasmid (m): sc-432328
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHDH CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHDH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CHDH Antibody (C-5): sc-393885
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHDH CRISPR/Cas9 KO Plasmid (m) | sc-432328 | 20 µg | $397.00 |
Overview
Chdh encodes choline dehydrogenase (CHDH), a mitochondrial inner membrane enzyme that catalyzes the oxidation of choline to betaine aldehyde, supporting downstream betaine production and one-carbon metabolism. Through its connection to methyl donor availability and osmolyte balance, CHDH influences phospholipid homeostasis, mitochondrial bioenergetics, and cellular responses to metabolic and oxidative stress. In mice, perturbation of choline oxidation can impact hepatic lipid handling and systemic energy balance, linking CHDH-associated pathways to metabolic phenotypes relevant to fatty liver and related cardiometabolic risk traits. These functions make CHDH a useful node for studying mitochondrial metabolism, methylation-dependent regulation, and nutrient–gene interactions in mammalian physiology.
CHDH CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chdh gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chdh together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chdh open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHDH protein expression.
This CRISPR knockout system enables efficient generation of Chdh-deficient cell models for investigation of CHDH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chdh exon(s) critical for CHDH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chdh genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHDH CRISPR/Cas9 KO Plasmid (m) and CHDH CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chdh locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHDH HDR Plasmid (m) and CHDH HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chdh homology arms to support homology-directed repair at defined Chdh target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.