CEL Antibody (D-1): sc-376956
- CEL Antibody (D-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 439-475 within an internal region of CEL of human origin
- recommended for detection of CEL long isoform of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CEL Antibody (D-1) for WB and IHC(P) applications. These reagents are now offered in bundles with CEL Antibody (D-1) (see ordering information below).
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CEL Antibody (D-1) is a mouse monoclonal IgG1 antibody that detects carboxyl ester lipase (CEL) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CEL plays a crucial role in lipid metabolism, particularly in the hydrolysis of cholesteryl esters, phospholipids, and acylglycerols, which are essential for maintaining cellular lipid homeostasis and energy balance. The enzyme′s active site features a catalytic triad composed of serine, histidine, and aspartate, which is vital for enzymatic activity. CEL is predominantly produced in the pancreas and lactating mammary gland, but can also be found in the liver, macrophages, and vascular walls, highlighting CEL′s importance in various physiological processes. CEL (D-1) antibody enables researchers to study CEL′s involvement in the assembly and secretion of chylomicrons, a key step in lipid transport, facilitated by CEL′s ability to hydrolyze ceramide. The diverse substrate reactivity of CEL suggests multiple functions in lipid and lipoprotein metabolism, as well as in the pathogenesis of atherosclerosis, making anti-CEL antibody (D-1) an invaluable tool for researchers studying these critical biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CEL Antibody (D-1) References:
- Carboxyl ester lipase: structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis. | Hui, DY. and Howles, PN. 2002. J Lipid Res. 43: 2017-30. PMID: 12454261
- Endocytosis of secreted carboxyl ester lipase in a syndrome of diabetes and pancreatic exocrine dysfunction. | Torsvik, J., et al. 2014. J Biol Chem. 289: 29097-111. PMID: 25160620
- Carboxyl-ester lipase maturity-onset diabetes of the young disease protein biomarkers in secretin-stimulated duodenal juice. | Bjorlykke, Y., et al. 2015. J Proteome Res. 14: 521-30. PMID: 25369532
- A Carboxyl Ester Lipase (CEL) Mutant Causes Chronic Pancreatitis by Forming Intracellular Aggregates That Activate Apoptosis. | Xiao, X., et al. 2016. J Biol Chem. 291: 23224-23236. PMID: 27650499
- The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. | Johansson, BB., et al. 2018. Pancreatology. 18: 12-19. PMID: 29233499
- Carboxyl Ester Lipase May Not Mediate Lipotoxic Injury during Severe Acute Pancreatitis. | Khatua, B., et al. 2019. Am J Pathol. 189: 1226-1240. PMID: 30954473
- Pathogenic Carboxyl Ester Lipase (CEL) Variants Interact with the Normal CEL Protein in Pancreatic Cells. | Dalva, M., et al. 2020. Cells. 9: PMID: 31963687
- Endoplasmic stress-inducing variants in carboxyl ester lipase and pancreatic cancer risk. | Kawamoto, M., et al. 2022. Pancreatology. 22: 959-964. PMID: 35995657
- Identification and characterization of novel carboxyl ester lipase gene variants in patients with different subtypes of diabetes. | Wu, H., et al. 2023. BMJ Open Diabetes Res Care. 11: PMID: 36634979
- Carboxyl ester lipase hybrid 1 (CEL-HYB1) haplotypes confer varying risk for chronic pancreatitis. | Berke, G., et al. 2024. Sci Rep. 14: 30965. PMID: 39730750
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEL Antibody (D-1) | sc-376956 | 200 µg/ml | $322.00 | |||
CEL Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-540673 | 200 µg Ab; 10 µg BP | $361.00 | |||
CEL Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-542288 | 200 µg Ab; 20 µg BP | $361.00 | |||
CEL (D-1) Neutralizing Peptide | sc-376956 P | 100 µg/0.5 ml | $69.00 |