CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (m): sc-419611
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDKN2B/p15 INK4B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDKN2B/p15 INK4B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PCLAF Antibody (G-11): sc-390515
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (m) | sc-419611 | 20 µg | $397.00 |
Overview
Mouse Cdkn2b encodes CDKN2B/p15 INK4B, a cyclin-dependent kinase inhibitor that restrains CDK4/6 activity to maintain RB-dependent control of the G1/S checkpoint. As a key effector downstream of anti-proliferative cues such as TGF-β signaling, p15 helps coordinate cell-cycle arrest, lineage differentiation programs, and responses to oncogenic stress. Disruption or epigenetic silencing of Cdkn2b is frequently associated with deregulated proliferation and is studied in the context of tumor suppressor networks and hematopoietic and epithelial transformation models. Cdkn2b function is commonly interrogated alongside the CDKN2A/B locus, CDK4/6–RB–E2F axis, and senescence-associated pathways.
CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdkn2b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdkn2b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdkn2b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDKN2B/p15 INK4B protein expression.
This CRISPR knockout system enables efficient generation of Cdkn2b-deficient cell models for investigation of CDKN2B/p15 INK4B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdkn2b exon(s) critical for CDKN2B/p15 INK4B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdkn2b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (m) and CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdkn2b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDKN2B/p15 INK4B HDR Plasmid (m) and CDKN2B/p15 INK4B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdkn2b homology arms to support homology-directed repair at defined Cdkn2b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.