CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (h2): sc-400570-KO-2

Overview

CDKN2B encodes p15 INK4B, a cyclin-dependent kinase inhibitor that binds CDK4/6 to restrain cyclin D–dependent phosphorylation of RB and enforce the G1/S checkpoint. Its expression is commonly induced by antiproliferative cues such as TGF-β signaling, linking extracellular stress and differentiation programs to cell-cycle arrest and senescence. CDKN2B is embedded in the 9p21 locus, a genomic region frequently altered in cancer and implicated in susceptibility to diverse age-related and cardiometabolic traits. Dysregulation of p15 INK4B–mediated CDK4/6 control contributes to aberrant proliferation, altered lineage commitment, and escape from growth-inhibitory pathways.

CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the CDKN2B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDKN2B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDKN2B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDKN2B/p15 INK4B protein expression.

This CRISPR knockout system enables efficient generation of CDKN2B-deficient cell models for investigation of CDKN2B/p15 INK4B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting CDKN2B exon(s) critical for CDKN2B/p15 INK4B function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple CDKN2B genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (h) and CDKN2B/p15 INK4B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDKN2B locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by CDKN2B/p15 INK4B HDR Plasmid (h) and CDKN2B/p15 INK4B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDKN2B homology arms to support homology-directed repair at defined CDKN2B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.