Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD97 CRISPR/Cas9 KO Plasmid (h2): sc-404492-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD97 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD97 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD97 Antibody (G-8): sc-166852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD97 CRISPR/Cas9 KO Plasmid (h2) | sc-404492-KO-2 | 20 µg | $397.00 |
Overview
ADGRE5 encodes CD97, an adhesion G protein-coupled receptor prominently expressed on leukocytes that mediates cell–cell and cell–matrix interactions. CD97 undergoes autoproteolytic processing and signals through heterotrimeric G proteins to influence cytoskeletal remodeling, migration, and immune cell positioning, integrating cues from extracellular matrix components and complement regulatory proteins. Through roles in adhesion and leukocyte trafficking, CD97 contributes to inflammatory microenvironment dynamics and has been studied in contexts of immune dysregulation and tumor–immune interactions. Its activity links adhesion-dependent signaling to downstream pathways that shape chemotaxis, tissue infiltration, and intercellular communication.
CD97 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the ADGRE5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADGRE5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADGRE5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD97 protein expression.
This CRISPR knockout system enables efficient generation of ADGRE5-deficient cell models for investigation of CD97 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADGRE5 exon(s) critical for CD97 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADGRE5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD97 CRISPR/Cas9 KO Plasmid (h) and CD97 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADGRE5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD97 HDR Plasmid (h) and CD97 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADGRE5 homology arms to support homology-directed repair at defined ADGRE5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.