CD63 Antibody (E-12): sc-365604
- CD63 Antibody (E-12) is a mouse monoclonal IgG2a κ, cited in 43 publications, provided at 200 µg/ml
- raised against amino acids 45-238 mapping at the C-terminus of CD63 of human origin
- CD63 Antibody (E-12) is recommended for detection of CD63 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CD63 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD63 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CD63 Antibody (E-12) (see ordering information below).
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CD63 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect CD63 protein of human origin. Anti-CD63 antibody (E-12) targets the epitope spanning amino acids 45-238 at the C-terminus of CD63 and is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. CD63, also known as LAMP-3, Melanoma-associated antigen ME491, and TSPAN30, is a member of the tetraspanin family of proteins characterized by four transmembrane domains, which are essential for the formation of multi-protein complexes involved in cell signaling and adhesion. The structural integrity provided by these transmembrane regions is crucial for CD63′s role in organizing membrane microdomains and interacting with specific integrins, thereby influencing cellular processes such as migration, proliferation, and immune responses. Predominantly located within lysosomal membranes, CD63 translocates to the plasma membrane upon cellular activation, such as during platelet activation, where CD63 plays a key role in phagocytosis and the fusion of lysosomes with phagosomes. This dynamic localization is vital for maintaining cellular homeostasis and effective immune function. CD63 is expressed on activated platelets, granulocytes, B cells, and T cells, highlighting CD63′s significant involvement in both innate and adaptive immune responses. Additionally, deficiencies in CD63 are associated with Hermansky-Pudlak syndrome, underscoring CD63′s crucial role in normal physiological processes. CD63 monoclonal antibody (E-12) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing researchers with versatile options to accommodate a wide range of experimental needs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD63 Antibody (E-12) | sc-365604 | 200 µg/ml | $322.00 | |||
CD63 Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-529404 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD63 Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-522287 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD63 Antibody (E-12): m-IgG2a BP-HRP Bundle | sc-547311 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD63 Antibody (E-12) AC | sc-365604 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD63 Antibody (E-12) HRP | sc-365604 HRP | 200 µg/ml | $322.00 | |||
CD63 Antibody (E-12) FITC | sc-365604 FITC | 200 µg/ml | $336.00 | |||
CD63 Antibody (E-12) PE | sc-365604 PE | 200 µg/ml | $349.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 488 | sc-365604 AF488 | 200 µg/ml | $364.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 546 | sc-365604 AF546 | 200 µg/ml | $364.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 594 | sc-365604 AF594 | 200 µg/ml | $364.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 647 | sc-365604 AF647 | 200 µg/ml | $364.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 680 | sc-365604 AF680 | 200 µg/ml | $364.00 | |||
CD63 Antibody (E-12) Alexa Fluor® 790 | sc-365604 AF790 | 200 µg/ml | $364.00 |