Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD30L CRISPR/Cas9 KO Plasmid (m): sc-423454
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD30L CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD30L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD30L Antibody (RM153): sc-19984
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD30L CRISPR/Cas9 KO Plasmid (m) | sc-423454 | 20 µg | $397.00 |
Overview
Tnfsf8 encodes CD30L (TNFSF8), a type II membrane ligand in the TNF superfamily that engages CD30 (TNFRSF8) to modulate immune cell communication. CD30L–CD30 signaling influences NF-κB and MAPK-dependent transcriptional programs that shape T cell costimulation, cytokine production, and activation-induced differentiation, and it can also affect cell survival decisions depending on cellular context. In mouse models, this axis is frequently studied in lymphoid tissue organization, germinal center responses, and regulation of inflammatory signaling networks relevant to immune dysregulation and lymphoproliferative phenotypes.
CD30L CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnfsf8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnfsf8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnfsf8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD30L protein expression.
This CRISPR knockout system enables efficient generation of Tnfsf8-deficient cell models for investigation of CD30L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnfsf8 exon(s) critical for CD30L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnfsf8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD30L CRISPR/Cas9 KO Plasmid (m) and CD30L CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnfsf8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD30L HDR Plasmid (m) and CD30L HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnfsf8 homology arms to support homology-directed repair at defined Tnfsf8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.