Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
casein kinase Iε Double Nickase Plasmid (h): sc-402163-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- casein kinase Iε Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- casein kinase Iε Double Nickase Plasmid (h) and casein kinase Iε Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSNK1E. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: casein kinase Iε Antibody (D-7): sc-365259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase Iε Double Nickase Plasmid (h) | sc-402163-NIC | 20 µg | $410.00 | |||
casein kinase Iε Double Nickase Plasmid (h2) | sc-402163-NIC-2 | 20 µg | $410.00 |
CSNK1E encodes human casein kinase Iε (CK1ε), a serine/threonine kinase that phosphorylates diverse substrates to coordinate circadian timing, receptor trafficking, and signal transduction. CK1ε is a key modulator of Wnt/β-catenin signaling through phosphorylation of Dishevelled and other pathway components, and it also contributes to DNA damage responses and cell-cycle control via phosphorylation-dependent protein turnover. Through these roles, CSNK1E influences transcriptional programs, protein stability, and cytoskeletal dynamics that shape proliferation and differentiation. Dysregulated CK1ε activity has been linked in the literature to altered circadian phenotypes and to signaling imbalances observed across multiple disease contexts, including cancer-related pathway rewiring.
casein kinase Iε Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSNK1E locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSNK1E. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSNK1E function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSNK1E-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.