C8A CRISPR/Cas9 KO Plasmid (h): sc-406590
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C8A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C8A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C8A CRISPR/Cas9 KO Plasmid (h) | sc-406590 | 20 µg | $397.00 |
Overview
C8A encodes the complement component 8 alpha chain, a core subunit of the terminal complement complex (C5b-9) that drives membrane attack complex (MAC) formation and target cell lysis. As part of the innate immune system, C8A participates in the complement cascade downstream of classical, lectin, and alternative pathway activation, influencing opsonization, inflammatory signaling, and immune complex clearance. Perturbation of terminal complement components is linked to dysregulated host defense and altered susceptibility to infection, and complement activity is also implicated in inflammatory and autoimmune-associated tissue injury. C8A is therefore relevant for studies of complement-mediated cytotoxicity, serum bactericidal activity, and mechanisms controlling immune homeostasis.
C8A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C8A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C8A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C8A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C8A protein expression.
This CRISPR knockout system enables efficient generation of C8A-deficient cell models for investigation of C8A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C8A exon(s) critical for C8A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C8A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C8A CRISPR/Cas9 KO Plasmid (h) and C8A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C8A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C8A HDR Plasmid (h) and C8A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C8A homology arms to support homology-directed repair at defined C8A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.