Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C5L2 CRISPR/Cas9 KO Plasmid (h): sc-410578
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C5L2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C5L2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C5L2 Antibody (E-8): sc-515734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C5L2 CRISPR/Cas9 KO Plasmid (h) | sc-410578 | 20 µg | $397.00 |
Overview
C5AR2 encodes C5L2, an atypical complement receptor that binds the anaphylatoxin C5a and C5a des-Arg, shaping complement-driven leukocyte signaling and inflammatory tone. Unlike the canonical C5aR1, C5L2 is often described as non–G protein-coupled and can modulate β-arrestin–linked signaling, receptor cross-talk, and downstream MAPK/NF-κB-associated responses in myeloid and tissue-resident cells. Through its role in complement and innate immune pathways, C5AR2 influences chemotaxis, cytokine production, and resolution programs that intersect with cardiometabolic inflammation and tissue injury responses. Altered C5AR2 activity or expression has been studied in contexts of sepsis-like systemic inflammation, atherosclerosis, and metabolic disorders, supporting its use as a mechanistic node in inflammatory disease models.
C5L2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C5AR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C5AR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C5AR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C5L2 protein expression.
This CRISPR knockout system enables efficient generation of C5AR2-deficient cell models for investigation of C5L2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C5AR2 exon(s) critical for C5L2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C5AR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C5L2 CRISPR/Cas9 KO Plasmid (h) and C5L2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C5AR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C5L2 HDR Plasmid (h) and C5L2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C5AR2 homology arms to support homology-directed repair at defined C5AR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.