Date published: 2025-9-8

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C3 Anticorpo (B-9): sc-28294

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Fichas de dados
  • C3 Anticorpo B-9é um anticorpo monoclonal produzido em camundongo IgG1 κ C3 anticorpo, citado em 60 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 541-840 de C3 precursor de human origem
  • C3 Anticorpo (B-9) é recomendado para a detecção de C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-C3 O anticorpo anti-C3 (B-9) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de C3 (B-9): sc-28294.
  • m-IgG Fc BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para C3 Anticorpo (B-9) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com C3 Anticorpo (B-9) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O anticorpo C3 (B-9) é um anticorpo monoclonal de cadeia leve IgG1 kappa de camundongo criado contra os aminoácidos 541-840 da proteína do componente 3 do complemento humano (C3). O C3, também conhecido como CPAMD1 ou proteína estimuladora da acilação (ASP), é um componente central do sistema do complemento, desempenhando um papel crucial na imunidade inata. Sintetizada principalmente no fígado, a C3 é segregada para a corrente sanguínea e existe no espaço extracelular, onde circula como um precursor inativo. Após ativação, o C3 é dividido em fragmentos de C3a e C3b; o C3a funciona como uma anafilatoxina que medeia as respostas inflamatórias, enquanto o C3b se liga às superfícies dos agentes patogénicos, promovendo a opsonização e a fagocitose pelas células imunitárias. A localização extracelular do C3 é essencial, uma vez que o C3 permite a interação imediata com agentes patogénicos invasores e a ativação da cascata do complemento nas superfícies celulares. A desregulação da ativação do C3 pode levar a doenças auto-imunes e a uma elevada suscetibilidade a infecções. O anticorpo C3 (B-9) reconhece o precursor C3, a anafilatoxina C3a, a cadeia α de C3, a cadeia β de C3 e a cadeia α′ de C3b de origem de camundongo, rato e humana, e é recomendado para utilização em western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF), imuno-histoquímica com secções incluídas em parafina (IHCP) e ensaio de imunoabsorção enzimática (ELISA). O anticorpo monoclonal C3 (B-9) é uma ferramenta valiosa para estudar as vias de ativação do complemento e as suas implicações nas respostas imunitárias em várias espécies.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do C3 Anticorpo (B-9):

  1. Modulação da atividade de C3a: internalização do recetor humano de C3a e sua inibição por C5a.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. A fosforilação induzida por ligandos dos receptores de anafilatoxinas C3aR e C5aR é mediada por 'cinases de receptores acoplados à proteína G'.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. Um papel para o recetor de anafilatoxina C3a na fase efectora da asma.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. Componente humano do complemento C3: sequência de codificação do cDNA e estrutura primária derivada.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. A ativação da proteína cinase activada por mitogénio requer duas entradas de sinal do recetor humano da anafilatoxina C5a.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  6. Expressão mediada por TNF-alfa do recetor da anafilatoxina C5a nos neurónios na meningoencefalite experimental por Listeria.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

C3 Anticorpo (B-9)

sc-28294
200 µg/ml
$316.00

Pacote do C3 (B-9): m-IgG Fc BP-HRP

sc-528410
200 µg Ab; 10 µg BP
$354.00

Pacote do C3 (B-9): m-IgGκ BP-HRP

sc-520784
200 µg Ab, 40 µg BP
$354.00

C3 Anticorpo (B-9) AC

sc-28294 AC
500 µg/ml, 25% agarose
$416.00

C3 Anticorpo (B-9) HRP

sc-28294 HRP
200 µg/ml
$316.00

C3 Anticorpo (B-9) FITC

sc-28294 FITC
200 µg/ml
$330.00

C3 Anticorpo (B-9) PE

sc-28294 PE
200 µg/ml
$343.00

C3 Anticorpo (B-9) Alexa Fluor® 488

sc-28294 AF488
200 µg/ml
$357.00

C3 Anticorpo (B-9) Alexa Fluor® 546

sc-28294 AF546
200 µg/ml
$357.00

C3 Anticorpo (B-9) Alexa Fluor® 594

sc-28294 AF594
200 µg/ml
$357.00

C3 Anticorpo (B-9) Alexa Fluor® 647

sc-28294 AF647
200 µg/ml
$357.00

C3 Anticorpo (B-9) Alexa Fluor® 680

sc-28294 AF680
200 µg/ml
$357.00

C3 Anticorpo (B-9) Alexa Fluor® 790

sc-28294 AF790
200 µg/ml
$357.00

Hello-Which antibody pairs would you recommend to develop a C3 sandwich ELISA

Asked by: Aaron Stoker
Thank you for your question. We have not validated our antibodies for sandwich ELISA.
Answered by: BlakeJ
Date published: 2024-09-20

hello,i see C3 antibody (B-9): sc-28294 is a mouse monoclonal antibody,how can it detect c3a anaphylatoxin and C3b α′ chain at the same time,if i want to use it in IHC,how can i distinguish it is C3b,C3a or c3?

Asked by: sophia
Thank you for your question. C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you would like to detect the specific protein, please select the other antibody. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2023-12-25

hello,can C3 Antibody (B-9): sc-28294 be used to dectect C3a anaphylatoxin in mouse.If so,did you have any such successful examples (western images) to shown to me ?

Asked by: sophia0314
Thank you for your question. Yes, C3 Antibody (B-9): sc-28294 is recommended for detection of C3a anaphylatoxin in mouse sample by WB, IP, IF, IHC(P) and ELISA. We don't have an in-house data for detection of C3a anaphylatoxin in mouse sample by WB at this moment, but please refer to the citations for this product.
Answered by: Technical Service SH
Date published: 2023-03-16

Can I use non-transfected HEK-293T as a negative control? In your legend of Near-infrared western blot analysis of C3 expression said 293T has C3 band. But same time in your "sc-28294 HRP. Direct western blot analysis" legend said 293T has no band.

Asked by: Woosung
Thank you for your question. Our Control 293T Lysate: sc-117752 is derived from non-transfected 293T cells and suitable for use as a negative control with our 293T Transfected Lysates. Our Control 293T Lysate goes through the same transfection process as our gene-specific transfected lysates, except without transfecting any cDNA into the cells. The Control 293T Lysate is also used at a lower concentration than regular cell lines for Western blotting. That said, it does appear that HEK293 does not have significant RNA expression of C3 per the Human Protein Atlas. We will review of the Near-infrared Western blot data showing a signal in HEK293T cell lysate, and if it can not be supported we will remove it.
Answered by: Technical Support
Date published: 2021-07-12

Has anyone used this AB for IHC in rat brain tissue? 

Asked by: Anonymous
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
Answered by: Tech Service
Date published: 2021-06-01

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

Asked by: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
Answered by: Technical Support
Date published: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

Asked by: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
Answered by: Technical Support
Date published: 2019-05-29

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

Asked by: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-04-12
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Rated 5 out of 5 by from Works well4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
Date published: 2018-08-01
Rated 1 out of 5 by from Does not detect human C3 in paraffin tissuesI tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
Date published: 2017-05-08
Rated 5 out of 5 by from Great for all manner of C3 detection.This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
Date published: 2017-01-05
Rated 5 out of 5 by from Superb!!!Great western if and ihc results; strong signal and low background
Date published: 2017-01-05
Rated 4 out of 5 by from IHC-Psome macrophages were positive for C3 by this antobody in IHC-P method
Date published: 2016-12-30
Rated 5 out of 5 by from Great in multiple applicationsThis antibody works very well in Western Blot, IF and IHC(P)
Date published: 2016-12-23
Rated 5 out of 5 by from I used it to detect mouse complement C3bI used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
Date published: 2015-10-15
Rated 5 out of 5 by from Great stain in mouse intestinal tissuesGreat stain in mouse intestinal tissues.
Date published: 2015-08-06
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C3 Anticorpo (B-9) is rated 4.7 out of 5 by 16.
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