Brn-3a/BRN3A/POU4F1 Antibody (H-6): sc-390078

Brn-3a Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects Brn-3a in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Brn-3a is a member of the Brn family of transcription factors, which play a pivotal role in the early embryonic development of the central nervous system by regulating gene expression in a highly restricted subset of neurons. Notably, Brn-3a contains two functional transactivating domains located at both the amino-terminus and carboxy-terminus, which are essential for stimulating transcription effectively. This structural feature is crucial as Brn-3a activates target genes necessary for neuronal differentiation and survival, thereby influencing the development of neural circuits. Additionally, Brn-3a′s interaction with other proteins, such as Brn-3b, which acts as a transcriptional repressor, highlights the complex regulatory networks that govern neuronal development. Anti-Brn-3a antibody (H-6) is an invaluable tool for researchers studying the mechanisms of transcriptional regulation in neurodevelopment and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Brn-3a/BRN3A/POU4F1 Antibody (H-6) References:

1. The BRN-3A transcription factor protects sensory but not sympathetic neurons from programmed cell death/apoptosis.  |  Ensor, E., et al. 2001. J Biol Chem. 276: 5204-12. PMID: 11053412
2. Brn-3a activates the expression of Bcl-x(L) and promotes neuronal survival in vivo as well as in vitro.  |  Smith, MD., et al. 2001. Mol Cell Neurosci. 17: 460-70. PMID: 11273642
3. The effects of Brn-3a on neuronal differentiation and apoptosis are differentially modulated by EWS and its oncogenic derivative EWS/Fli-1.  |  Gascoyne, DM., et al. 2004. Oncogene. 23: 3830-40. PMID: 15021903
4. Coexpression of Brn-3a POU protein with p53 in a population of neuronal progenitor cells is associated with differentiation and protection against apoptosis.  |  Hudson, CD., et al. 2004. J Neurosci Res. 78: 803-14. PMID: 15532030
5. Brn-3a neuronal transcription factor functional expression in human prostate cancer.  |  Diss, JK., et al. 2006. Prostate Cancer Prostatic Dis. 9: 83-91. PMID: 16276351
6. Cardiac expression of Brn-3a and Brn-3b POU transcription factors and regulation of Hsp27 gene expression.  |  Farooqui-Kabir, SR., et al. 2008. Cell Stress Chaperones. 13: 297-312. PMID: 18368538
7. Brn-3a/POU4F1 interacts with and differentially affects p73-mediated transcription.  |  Hudson, CD., et al. 2008. Cell Death Differ. 15: 1266-78. PMID: 18421303
8. Regulation of Brn-3a N-terminal transcriptional activity by MEK1/2-ERK1/2 signalling in neural differentiation.  |  Berwick, DC., et al. 2009. Brain Res. 1256: 8-18. PMID: 19135033
9. The cellular transcription factor Brn-3a and the smoking-related substance nicotine interact to regulate the activity of the HPV URR in the cervix.  |  Ndisang, D., et al. 2010. Oncogene. 29: 2701-11. PMID: 20190800
10. POU transcription factors Brn-3a and Brn-3b interact with the estrogen receptor and differentially regulate transcriptional activity via an estrogen response element.  |  Budhram-Mahadeo, V., et al. 1998. Mol Cell Biol. 18: 1029-41. PMID: 9448000

The preferred Brn-3a/BRN3A/POU4F1 antibody is Brn-3a/BRN3A/POU4F1 Antibody (14A6): sc-8429.