Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BRAP Double Nickase Plasmid (h): sc-403847-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BRAP Double Nickase Plasmid (h) and BRAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BRAP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRAP Antibody (D-5): sc-166012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRAP Double Nickase Plasmid (h) | sc-403847-NIC | 20 µg | $410.00 | |||
BRAP Double Nickase Plasmid (h2) | sc-403847-NIC-2 | 20 µg | $410.00 |
BRAP (BRCA1 associated protein) is a cytoplasmic E3 ubiquitin ligase that modulates MAPK signaling by binding and ubiquitinating components such as KSR1, thereby shaping RAF–MEK–ERK pathway amplitude and duration. Through ubiquitin-dependent control of protein stability and trafficking, BRAP influences cell-cycle progression, differentiation, and stress-responsive signaling. Genetic and functional studies have linked BRAP perturbation to altered proliferative signaling and dysregulated proteostasis, processes relevant to cancer biology and neurovascular disease mechanisms. Its role at the intersection of ubiquitination and MAPK pathway regulation makes BRAP a useful node for dissecting signal transduction and protein turnover networks in human cells.
BRAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BRAP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BRAP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BRAP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BRAP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.