Bag-1 Antibody (CC9E8): sc-33704

Bag-1 Antibody (CC9E8) is a mouse monoclonal IgG1 light chain antibody that detects Bag-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Bag-1 plays a crucial role in regulating apoptosis by enhancing the protective effects of Bcl-2 against cell death, which is vital for maintaining cellular homeostasis and preventing unwanted cell loss. Bag-1 is primarily located in the cytoplasm, where Bag-1 interacts with various proteins, including Bcl-2, to modulate apoptotic signaling pathways. The ability of Bag-1 to bind to Bcl-2 suggests that Bag-1 may serve as a critical factor in determining cell fate, particularly in contexts where apoptosis needs to be tightly regulated, such as during development or in response to stress. By understanding the interactions and functions of Bag-1, researchers can gain insights into the mechanisms of cell survival and death, which are essential for developing therapeutic strategies for diseases characterized by dysregulated apoptosis, such as cancer. Anti-Bag-1 antibody (CC9E8) is an invaluable tool for studying these complex processes in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bag-1 Antibody (CC9E8) References:

1. BAG-1 interacts with the p50-p50 homodimeric NF-κB complex: implications for colorectal carcinogenesis.  |  Southern, SL., et al. 2012. Oncogene. 31: 2761-72. PMID: 21963853
2. BAG-1 stabilizes mutant F508del-CFTR in a ubiquitin-like-domain-dependent manner.  |  Mendes, F., et al. 2012. Cell Physiol Biochem. 30: 1120-33. PMID: 23178238
3. Targeting BAG-1: a novel strategy to increase drug efficacy in acute myeloid leukemia.  |  Aveic, S., et al. 2015. Exp Hematol. 43: 180-190.e6. PMID: 25461257
4. Regulation of osteoblast development by Bcl-2-associated athanogene-1 (BAG-1).  |  Greenhough, J., et al. 2016. Sci Rep. 6: 33504. PMID: 27633857
5. Bag-1 silencing enhanced chemotherapeutic drug-induced apoptosis in MCF-7 breast cancer cells affecting PI3K/Akt/mTOR and MAPK signaling pathways.  |  Kilbas, PO., et al. 2019. Mol Biol Rep. 46: 847-860. PMID: 30661182
6. Inhibition of BAG-1 induced SH-SY5Y cell apoptosis without affecting Hsp70 expression.  |  Song, YK., et al. 2020. J Cell Biochem. 121: 1728-1735. PMID: 31609014
7. Bag-1 Silence Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin Through Multiple Gene Pathways.  |  Lv, J., et al. 2019. Onco Targets Ther. 12: 8977-8989. PMID: 31802907
8. Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer.  |  Kizilboga, T., et al. 2019. BMC Cancer. 19: 1254. PMID: 31883527
9. Mutation of the BAG-1 domain decreases its protective effect against hypoxia/reoxygenation by regulating HSP70 and the PI3K/AKT signalling pathway in SY-SH5Y cells.  |  Chen, Y., et al. 2021. Brain Res. 1751: 147192. PMID: 33152339
10. Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation.  |  Can, ND., et al. 2021. PLoS One. 16: e0256640. PMID: 34428256

The preferred Bag-1 antibody is Bag-1 Antibody (3.10G3E2): sc-56003.