APOBEC3B Double Nickase Plasmid (h): sc-401700-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APOBEC3B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APOBEC3B Double Nickase Plasmid (h) and APOBEC3B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APOBEC3B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APOBEC3B Double Nickase Plasmid (h) | sc-401700-NIC | 20 µg | $410.00 | |||
APOBEC3B Double Nickase Plasmid (h2) | sc-401700-NIC-2 | 20 µg | $410.00 |
APOBEC3B (apolipoprotein B mRNA editing enzyme catalytic subunit 3B) is a single-stranded DNA cytidine deaminase that converts cytosine to uracil during DNA replication and repair, generating characteristic C→T/G mutational signatures. It functions in intrinsic antiviral defense and intersects with replication stress responses, DNA damage signaling, and mutagenic repair processes that shape genome stability. Dysregulated APOBEC3B activity has been associated with elevated somatic mutation burdens and tumor evolution across multiple cancer types, making it a key factor in studies of mutagenesis, clonal diversification, and genome maintenance pathways. Researchers commonly investigate APOBEC3B in the context of interferon-regulated innate immunity, nucleic acid metabolism, and mechanisms that couple replication-associated ssDNA exposure to editing activity.
APOBEC3B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APOBEC3B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APOBEC3B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APOBEC3B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APOBEC3B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.