Angiopoietin 1/Ang-1/ANGPT1 CRISPR/Cas9 KO Plasmid (m): sc-419041

Overview

Angiopoietin 1 (ANGPT1; Ang-1) is a secreted vascular growth factor that binds the endothelial receptor tyrosine kinase TIE2/TEK to promote vessel maturation, endothelial survival, and barrier stabilization. In mouse tissues, ANGPT1 signaling coordinates pericyte recruitment, extracellular matrix remodeling, and anti-permeability responses, counterbalancing destabilizing cues during angiogenic sprouting. This pathway contributes to regulation of vascular quiescence and inflammatory cell trafficking by modulating junctional integrity and endothelial activation programs. Dysregulation of ANGPT1–TIE2 signaling has been implicated in pathological angiogenesis and vascular leakage phenotypes relevant to tumor microenvironment remodeling, ischemic injury models, and inflammatory vascular disease research.

Angiopoietin 1/Ang-1/ANGPT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Angpt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Angpt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Angpt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Angiopoietin 1/Ang-1/ANGPT1 protein expression.

This CRISPR knockout system enables efficient generation of Angpt1-deficient cell models for investigation of Angiopoietin 1/Ang-1/ANGPT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Angpt1 exon(s) critical for Angiopoietin 1/Ang-1/ANGPT1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Angpt1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Angiopoietin 1/Ang-1/ANGPT1 CRISPR/Cas9 KO Plasmid (m) and Angiopoietin 1/Ang-1/ANGPT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Angpt1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Angiopoietin 1/Ang-1/ANGPT1 HDR Plasmid (m) and Angiopoietin 1/Ang-1/ANGPT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Angpt1 homology arms to support homology-directed repair at defined Angpt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.