Date published: 2025-9-8

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Alix Anticorpo (2H12): sc-53539

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Fichas de dados
  • Alix Anticorpo 2H12é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 11 publicações, fornecido em 200 µg/ml
  • recomendado para a detecção de Alix de mouse, rat, human e Xenopus de origem WB, IP, IF e ELISA
  • See Alix (1A12): sc-53540 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para Alix Antibody (2H12) para aplicações WB. Estes reagentes são agora oferecidos em pacotes com Alix Antibody (2H12)(ver informações de encomenda abaixo).

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VEJA TAMBÉM

A ALG-2-interacting protein (Alix), também designada como programmed cell death 6-interacting protein (PDCD6-interacting protein), é uma proteína citoplasmática. A Alix interage com proteínas associadas à apoptose (ALG-2 e PDCD6) e com o regulador de endocitose CIN85. Além disso, a Alix interage com os complexos de classificação endossômica necessários para as proteínas de transporte (ESCRT) (Tsg101 e CHMP4) e pode se associar ao HIV-1. As endofilinas (SH3P4, SH3P8 e SH3P13), enzimas que alteram a curvatura da membrana e que são necessárias para as etapas iniciais e finais da formação de vesículas revestidas, também se ligam à Alix. A Alix está envolvida na concentração e classificação de proteínas de carga do corpo multivesicular para incorporação em vesículas.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Alix Anticorpo (2H12)

sc-53539
200 µg/ml
$316.00

Pacote do Alix (2H12): m-IgG Fc BP-HRP

sc-536983
200 µg Ab; 10 µg BP
$354.00

Pacote do Alix (2H12): m-IgGκ BP-HRP

sc-534162
200 µg Ab; 40 µg BP
$354.00

Pacote do Alix (2H12): m-IgG1 BP-HRP

sc-544939
200 µg Ab; 20 µg BP
$354.00

Are there any issues with using sc-53539: Alix (2H12) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53539 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

What is the lower mw band with the nuclear extract.   Is that an Alix isoform or contaminate ?

Asked by: Roger Madison
Thank you for your question. We have not further characterized the lower molecular weight band observed in the nuclear extracts tested (HeLa, Jurkat, K-562, THP-1 and U-937) by Western blot with this Alix Antibody (2H12): sc-53539. However, this band is also observed with a different Alix antibody with a different epitope, sc-53540: Alix (1A12).
Answered by: Technical Support
Date published: 2016-12-20
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Rated 5 out of 5 by from Good antibody for WBThe Alix Antibody (2H12): sc-53539 works well for Western blot applications. This antibody has also been cited several times for further verification.
Date published: 2017-01-18
Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-04-28
Rated 4 out of 5 by from Publishable data in WB using mouse brainPublishable data in WB using mouse brain tumor exosomes -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Bands seen in WB using HeLaBands seen in WB using HeLa, Jurkat, K-562, THP-1 and U-937 nuclear extracts. -SCBT QC
Date published: 2015-01-10
Rated 4 out of 5 by from Publishable data in IP using mouse C2C12(myoblastPublishable data in IP using mouse C2C12(myoblast cell line) -SCBT Publication Review
Date published: 2013-01-24
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Alix Anticorpo (2H12) is rated 4.4 out of 5 by 5.
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