Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ADRP CRISPR/Cas9 KO Plasmid (h): sc-400802
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADRP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADRP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADRP Antibody (B-6): sc-377429
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADRP CRISPR/Cas9 KO Plasmid (h) | sc-400802 | 20 µg | $397.00 |
Overview
PLIN2 encodes adipose differentiation-related protein (ADRP), a lipid droplet coat protein that regulates neutral lipid storage and the dynamics of lipid droplet biogenesis, expansion, and turnover. ADRP coordinates interactions between lipid droplets and cellular membranes and influences metabolic processes including fatty acid handling, lipotoxic stress responses, and organelle crosstalk in hepatocytes, adipocytes, and macrophages. Through its role in intracellular lipid trafficking and storage, PLIN2 is commonly studied in the context of hepatic steatosis, obesity-associated metabolic dysfunction, and foam cell formation during atherogenesis. Altered PLIN2 expression is also investigated in cancers with lipid droplet–rich phenotypes, where lipid storage programs can support proliferation and oxidative stress adaptation.
ADRP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLIN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLIN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLIN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADRP protein expression.
This CRISPR knockout system enables efficient generation of PLIN2-deficient cell models for investigation of ADRP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLIN2 exon(s) critical for ADRP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLIN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADRP CRISPR/Cas9 KO Plasmid (h) and ADRP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLIN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADRP HDR Plasmid (h) and ADRP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLIN2 homology arms to support homology-directed repair at defined PLIN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.