ADH5 Double Nickase Plasmid (m): sc-419007-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADH5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADH5 Double Nickase Plasmid (m) and ADH5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adh5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADH5 Double Nickase Plasmid (m) | sc-419007-NIC | 20 µg | $410.00 | |||
ADH5 Double Nickase Plasmid (m2) | sc-419007-NIC-2 | 20 µg | $410.00 |
Mouse Adh5 encodes alcohol dehydrogenase 5 (ADH5), a widely expressed cytosolic dehydrogenase that functions as S-nitrosoglutathione reductase to regulate nitric oxide bioactivity by metabolizing S-nitrosoglutathione and related formaldehyde-derived adducts. Through control of protein S-nitrosylation and redox balance, ADH5 influences oxidative and nitrosative stress responses, glutathione-dependent metabolism, and downstream signaling pathways affecting mitochondrial function and inflammatory programs. Perturbation of ADH5 activity has been linked to altered detoxification capacity and dysregulated NO signaling, with implications for models of liver injury, immune dysfunction, and neurodegenerative processes where reactive carbonyl and nitrogen species accumulate. Adh5 therefore provides a tractable target for studying stress-adaptation networks and redox-sensitive regulation of cellular homeostasis in mouse systems.
ADH5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adh5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adh5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adh5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adh5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.