Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h): sc-403213-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h) and Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY7. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h) | sc-403213-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h2) | sc-403213-NIC-2 | 20 µg | $410.00 |
ADCY7 encodes adenylate cyclase 7 (AC7), a membrane-associated enzyme that catalyzes ATP conversion to cyclic AMP (cAMP), a central second messenger controlling PKA- and EPAC-dependent signaling. AC7 activity is regulated downstream of GPCRs via heterotrimeric G proteins, linking extracellular cues to transcriptional programs, cytokine signaling, and innate immune cell activation. Through modulation of cAMP dynamics, ADCY7 contributes to pathways governing inflammatory responses, cellular stress signaling, and metabolic regulation. Genetic variation or dysregulated expression of ADCY7 has been associated with altered immune homeostasis and inflammatory disease susceptibility, motivating mechanistic studies in relevant human cell models.
Adenylate cyclase 7/AC7/ADCY7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.