Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
adenosine deaminase CRISPR/Cas9 KO Plasmid (h): sc-402201
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- adenosine deaminase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the adenosine deaminase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: adenosine deaminase Antibody (D-4): sc-28346
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
adenosine deaminase CRISPR/Cas9 KO Plasmid (h) | sc-402201 | 20 µg | $397.00 |
Overview
Human ADA encodes adenosine deaminase, a cytosolic enzyme that catalyzes deamination of adenosine and deoxyadenosine to inosine derivatives, maintaining purine homeostasis and regulating intracellular deoxyadenosine nucleotide pools. By controlling levels of immunomodulatory adenosine and potentially cytotoxic deoxyadenosine metabolites, ADA influences lymphocyte development, proliferation, and signaling networks linked to cellular stress and metabolic control. ADA activity intersects with purine salvage and catabolic pathways, shaping nucleotide balance, DNA synthesis capacity, and adenosine receptor–mediated signaling in immune and non-immune tissues. Genetic disruption of ADA is associated with severe immunodeficiency phenotypes and has been used broadly to study purinergic metabolism, immune cell viability, and metabolite-driven regulation of gene expression.
adenosine deaminase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish adenosine deaminase protein expression.
This CRISPR knockout system enables efficient generation of ADA-deficient cell models for investigation of adenosine deaminase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADA exon(s) critical for adenosine deaminase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by adenosine deaminase CRISPR/Cas9 KO Plasmid (h) and adenosine deaminase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by adenosine deaminase HDR Plasmid (h) and adenosine deaminase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADA homology arms to support homology-directed repair at defined ADA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.