Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACLP/AEBP1 CRISPR/Cas9 KO Plasmid (h): sc-403540
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACLP/AEBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACLP/AEBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACLP/AEBP1 Antibody (G-1): sc-271374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACLP/AEBP1 CRISPR/Cas9 KO Plasmid (h) | sc-403540 | 20 µg | $397.00 |
Overview
AEBP1 encodes adipocyte enhancer-binding protein 1, also known as ACLP, an extracellular matrix–associated protein implicated in collagen organization, cell–matrix adhesion, and tissue remodeling. In stromal and fibroblast-like contexts, AEBP1 is linked to pathways that regulate mesenchymal activation, inflammatory signaling, and extracellular matrix turnover, influencing cellular migration and mechanical properties of tissues. Altered AEBP1/ACLP expression has been associated with fibrotic remodeling and tumor microenvironment biology, where it can correlate with changes in stromal composition and immune-related signaling. These features make AEBP1 a useful target for mechanistic studies of matrix-driven phenotypes and cell state transitions.
ACLP/AEBP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AEBP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AEBP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AEBP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACLP/AEBP1 protein expression.
This CRISPR knockout system enables efficient generation of AEBP1-deficient cell models for investigation of ACLP/AEBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AEBP1 exon(s) critical for ACLP/AEBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AEBP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACLP/AEBP1 CRISPR/Cas9 KO Plasmid (h) and ACLP/AEBP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AEBP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACLP/AEBP1 HDR Plasmid (h) and ACLP/AEBP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AEBP1 homology arms to support homology-directed repair at defined AEBP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.