ACCα Antibody (D-5): sc-137104
- ACCα Antibody (D-5) is a mouse monoclonal IgG2b κ ACCα antibody, cited in 30 publications, provided at 200 µg/ml
- raised against amino acids 1-76 mapping at the N-terminus of ACCα of human origin
- ACC alpha Antibody (D-5) is recommended for detection of ACCα of human origin by WB, IP, IF and ELISA
- Anti-ACC alpha Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACCα Antibody (D-5) for WB applications. These reagents are now offered in bundles with ACCα Antibody (D-5) (see ordering information below).
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ACCα Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACCα protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACCα (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Acetyl-CoA carboxylase (ACC) plays a crucial role in lipid metabolism, catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid synthesis. This enzymatic activity regulates the production of long-chain fatty acids, essential for energy storage and membrane synthesis. ACCα isoform, also known as ACC1, is predominantly expressed in lipogenic tissues and regulated by phosphorylation and dephosphorylation of specific serine residues, as well as by allosteric interactions with metabolites such as citrate and palmitoyl-CoA. These regulatory mechanisms ensure fatty acid synthesis is appropriately modulated in response to cellular energy demands and nutritional status. Located on human chromosome 17, ACCα plays a significant role in metabolic pathways. Understanding ACCα function and regulation is vital for elucidating its role in metabolic disorders, including obesity and diabetes, making anti-ACCα antibody (D-5) an essential tool for researchers studying lipid metabolism and related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACCα Antibody (D-5) References:
- The subcellular localization of acetyl-CoA carboxylase 2. | Abu-Elheiga, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 1444-9. PMID: 10677481
- Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. | Dean, D., et al. 2000. Diabetes. 49: 1295-300. PMID: 10923628
- Cloning of human acetyl-CoA carboxylase beta promoter and its regulation by muscle regulatory factors. | Lee, JJ., et al. 2001. J Biol Chem. 276: 2576-85. PMID: 11076940
- Activation of acetyl-CoA carboxylase by a glutamate- and magnesium-sensitive protein phosphatase in the islet beta-cell. | Kowluru, A., et al. 2001. Diabetes. 50: 1580-7. PMID: 11423479
- Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility. | Sinilnikova, OM., et al. 2004. Carcinogenesis. 25: 2417-24. PMID: 15333468
- RNA interference-mediated silencing of the acetyl-CoA-carboxylase-alpha gene induces growth inhibition and apoptosis of prostate cancer cells. | Brusselmans, K., et al. 2005. Cancer Res. 65: 6719-25. PMID: 16061653
- Acetyl-CoA carboxylase alpha is essential to breast cancer cell survival. | Chajès, V., et al. 2006. Cancer Res. 66: 5287-94. PMID: 16707454
- Acetyl-CoA carboxylase-alpha inhibitor TOFA induces human cancer cell apoptosis. | Wang, C., et al. 2009. Biochem Biophys Res Commun. 385: 302-6. PMID: 19450551
- Involvement of ACACA (acetyl-CoA carboxylase α) in the lung pre-metastatic niche formation in breast cancer by senescence phenotypic conversion in fibroblasts. | Huang, YC., et al. 2023. Cell Oncol (Dordr). 46: 643-660. PMID: 36607556
- Identification of acetyl-CoA carboxylase alpha as a prognostic and targeted candidate for hepatocellular carcinoma. | Shen, Y., et al. 2023. Clin Transl Oncol. 25: 2499-2513. PMID: 36976490
- Nicotinate-curcumin improves NASH by inhibiting the AKR1B10/ACCα-mediated triglyceride synthesis. | Lin, XL., et al. 2024. Lipids Health Dis. 23: 201. PMID: 38937844
- Regulation of mammalian acetyl-coenzyme A carboxylase. | Kim, KH. 1997. Annu Rev Nutr. 17: 77-99. PMID: 9240920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACCα Antibody (D-5) | sc-137104 | 200 µg/ml | $322.00 | |||
ACCα Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-521427 | 200 µg Ab, 40 µg BP | $361.00 | |||
ACCα Antibody (D-5): m-IgG2b BP-HRP Bundle | sc-548895 | 200 µg Ab; 10 µg BP | $361.00 | |||
ACCα Antibody (D-5) AC | sc-137104 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ACCα Antibody (D-5) HRP | sc-137104 HRP | 200 µg/ml | $322.00 | |||
ACCα Antibody (D-5) FITC | sc-137104 FITC | 200 µg/ml | $336.00 | |||
ACCα Antibody (D-5) PE | sc-137104 PE | 200 µg/ml | $349.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 488 | sc-137104 AF488 | 200 µg/ml | $364.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 546 | sc-137104 AF546 | 200 µg/ml | $364.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 594 | sc-137104 AF594 | 200 µg/ml | $364.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 647 | sc-137104 AF647 | 200 µg/ml | $364.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 680 | sc-137104 AF680 | 200 µg/ml | $364.00 | |||
ACCα Antibody (D-5) Alexa Fluor® 790 | sc-137104 AF790 | 200 µg/ml | $364.00 |