β-casein Double Nickase Plasmid (h): sc-402248-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β-casein Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- β-casein Double Nickase Plasmid (h) and β-casein Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β-casein Antibody (F20.14): sc-53189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-casein Double Nickase Plasmid (h) | sc-402248-NIC | 20 µg | $410.00 | |||
β-casein Double Nickase Plasmid (h2) | sc-402248-NIC-2 | 20 µg | $410.00 |
CSN2 encodes human β-casein, a major secreted phosphoprotein of milk that contributes to micelle formation and facilitates delivery of calcium, phosphate, and bioactive peptides during lactation. Its expression is tightly regulated in mammary epithelium by lactogenic hormone signaling, prominently the prolactin–JAK2/STAT5 axis, with additional input from glucocorticoid receptor and insulin/PI3K pathways that coordinate differentiation and secretory function. Altered β-casein regulation is widely used as a readout of mammary epithelial differentiation state and lactation-associated transcriptional programs, making CSN2 a useful molecular marker in studies of mammary gland development and endocrine control of gene expression. Dysregulated milk-protein gene networks, including CSN2, are frequently examined in contexts such as impaired lactation physiology and shifts in epithelial lineage programs relevant to breast biology research.
β-casein Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.